Background
Previous cohort studies have associated lower testosterone concentrations with poorer cognitive function, and higher dementia risk, with clinical trials of testosterone providing inconclusive results.
Aims
To clarify whether endogenous concentrations of testosterone, and of other sex hormones, were associated with cognition and incident dementia in men.
Methods
A systematic review was conducted, identifying suitable studies from which individual participant data (IPD) were requested (PROSPERO registration: CRD42019139668). Eligible studies were prospective cohort studies measuring testosterone using mass spectrometry, with at least five years of follow-up data. IPD meta-analysis (IPDMA) models were fitted. Datasets that included both the outcome (baseline cognitive function or time to dementia diagnosis) and exposure (total testosterone [T] or sex hormone-binding globulin [SHBG] concentration) were analysed.
Results
The cross-sectional analysis included 8,848 men, median age 74 years, from 6 cohorts; the longitudinal analysis had median 12.7 years and total 122,493 participant-years of follow-up. Harmonised z-scores of global cognition were not associated with T (estimated mean difference [MD] at medians of quintile 1 [Q1] vs Q5, 7.8 vs 22.6 nmol/L; MD, 0.04, 95% CI, -0.05 to 0.14) or with SHBG (26.5 [Q1] vs. 76.9 [Q5] nmol/L; MD, 0.08 [CI, -0.01 to 0.18]). Incident dementia was not associated with T (adjusted hazard ratio, HR for Q1 vs Q5, 0.97, [CI 0.76 to 1.23]) but men with lower SHBG had an estimated lower risk of incident dementia, relative to men with reference level SHBG (Q1 vs Q5; adjusted HR, 0.80 [CI 0.67 to 0.96]). The maximum of relative heterogeneity estimates was an I2 of 5.0, CI 0.0 to 53.6.
Conclusions
Constraining study eligibility to those that assayed testosterone using mass spectrometry facilitated analyses of high-quality datasets, using multivariable non-linear models. Lower SHBG is a biomarker for reduced risk of incident dementia in men, warranting further studies to explore potential mechanisms.